Asante Kotoko SC joined a lengthy list of domestic Cup casualties to compound their woes in the latest surprises from the annual cup competition.
The Porcupine Warriors suffered a huge blow in their FA Cup dreams with a damning 1-2 home defeat at the hands of Asokwa Deportivo in the round of 64.
The humiliation defeat ended their reign as defending champions of the MTN FA Cup after a marathon run of 848 days.
Like typical FA Cup competitions across the country, the cup casualties included five other Premier League teams including Inter Allies FC and Berekum Chelsea FC.
With 64 clubs battling for a place in the round of 32, Asante Kotoko SC led the list of victims in the annual elimination cup.
Ebusua Dwarfs, Berekum Chelsea FC, Inter Allies FC, Techiman Eleven Wonders FC, Aduana Stars and the Porcupine Warriors all failed to skip past the first hurdles in the MTN FA Cup.
Legon Cities FC nailed Inter Allies FC 3-0 in the capital Showdown whiles Unity FC proved too strong for Berekum Chelsea FC to contain.
Aduana Stars lost on home soil to Bechem United FC in a painful 1-2 reverse to end their Cup dreams as well.
The lottery of shootouts evicted Ebusua Dwarfs after a thrilling 90 minutes in the cup clash packed with end to end game.
Asante Kotoko SC will now have to win the league at the end of the season to make a return to African football.